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primescripttm rt mastermix kit  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm rt mastermix kit
    Primescripttm Rt Mastermix Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 26693 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+rt+mastermix+kit/PrimeScript+RT+Master+Mix/pm38785143-80-25-29
    Average 99 stars, based on 26693 article reviews
    primescripttm rt mastermix kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Circular RNA hsa_circ_0049657 as a Potential Biomarker in Non-Small Cell Lung Cancer.
    Article Snippet: RNA concentration was measured using an ultraviolet spectrophotometer (IMPLEN, Germany). .. Subsequently, extracted RNA was reverse transcribed into cDNA according to the instructions of the PrimeScriptTM RT MasterMix kit (Takara, Dalian, China). .. PCRs were performed by using the SYBR Green method in an ABI 7500 FAST sequence detection system (Applied Biosystems).

    Article Title: Hydrogen sulfide attenuates mitochondrial dysfunction-induced cellular senescence and apoptosis in alveolar epithelial cells by upregulating sirtuin 1
    Article Snippet: At the end of incubation, the cells were microscopically examined at 200× magnification (Leica, Germany). .. Total RNA was extracted from A549 cells using Trizol reagent (Invitrogen Corporation, CA, USA) followed by reverse transcription using a PrimeScriptTM RT MasterMix Kit (Takara, Shiga, Japan). .. Real-time PCR was performed with the above cDNA using SYBR Green Fast qPCR mix (Takara) with an iCyler iQ Real-time PCR Detection System (Bio-Rad Laboratories Inc., USA).

    Article Title: Inhibition of Arginyl-tRNA Synthetase Promotes the Protection of Neuronal Ischemic Tolerance in Vitro Author Imformation
    Article Snippet: .. RNA was reverse transcribed to cDNA using a PrimeScriptTM RT MasterMix Kit (Takara Corporation). ..

    Synthesized:

    Article Title: Astragaloside IV protects against autoimmune myasthenia gravis in rats via regulation of mitophagy and apoptosis.
    Article Snippet: .. Total rna was obtained from the gastrocnemius muscle tissue samples of rats using Trnzol‐a+ (Tiangen Biotech co., ltd.). cdna was synthesized by rT using a PrimeScriptTM rT MasterMix Kit (Takara Bio, inc.). .. The expression of target genes was quantified by qPCR with TB GreenTM Premix Ex TaqTM II (Takara Bio, inc.) using a cFX96 real‐Time Pcr System (Bio‐rad laboratories, inc.).

    Article Title: GPAA1 promotes the proliferation, invasion and migration of hepatocellular carcinoma cells by binding to RNA-binding protein SF3B4
    Article Snippet: .. Total RNA was extracted from MIHA, Hep10, HuH-7, SNU-387 and Hep3B cells using TRIzol ® (Invitrogen; Thermo Fisher Scientific, Inc.) and cDNA was synthesized using the PrimeScriptTM RT MasterMix kit (Takara Bio, Inc.) according to the manufacturer's protocol. qPCR was performed using the LightCycler 480 Probes Master kit (Roche Applied Science) on a LightCycler 480 system (Roche Applied Science). ..

    Real-time Polymerase Chain Reaction:

    Article Title: GPAA1 promotes the proliferation, invasion and migration of hepatocellular carcinoma cells by binding to RNA-binding protein SF3B4
    Article Snippet: .. Total RNA was extracted from MIHA, Hep10, HuH-7, SNU-387 and Hep3B cells using TRIzol ® (Invitrogen; Thermo Fisher Scientific, Inc.) and cDNA was synthesized using the PrimeScriptTM RT MasterMix kit (Takara Bio, Inc.) according to the manufacturer's protocol. qPCR was performed using the LightCycler 480 Probes Master kit (Roche Applied Science) on a LightCycler 480 system (Roche Applied Science). ..

    Article Title: CYB561 promotes HER2+ breast cancer proliferation by inhibiting H2AFY degradation.
    Article Snippet: Total RNA was extracted by TRIzol reagent (Ambion, USA). .. Nest, reversed transcription of RNA to cDNA using the PrimeScriptTM RT MasterMix kit (Takara, Japan). qRT-PCR was performed using AceQ SYBR qPCR Master Mix (Vazyme, China) according to the manufacturer’s instructions. ..

    Quantitative RT-PCR:

    Article Title: CYB561 promotes HER2+ breast cancer proliferation by inhibiting H2AFY degradation.
    Article Snippet: Total RNA was extracted by TRIzol reagent (Ambion, USA). .. Nest, reversed transcription of RNA to cDNA using the PrimeScriptTM RT MasterMix kit (Takara, Japan). qRT-PCR was performed using AceQ SYBR qPCR Master Mix (Vazyme, China) according to the manufacturer’s instructions. ..



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